We determined the mRNA expression profiles of GSTCD, HHIP, THSD4, TNS1, HTR4, AGER and NOTCH4 in human lung tissue and a series of primary cells. We detected all transcripts in lung tissue (Supplementary Fig. 2a) and bronchial epithelial cells (Supplementary Fig. 2b); six transcripts (excluding NOTCH4) were present in human airway smooth muscle cells. We also detected GSTCD, TNS1, HTR4, AGER and NOTCH4 transcripts in peripheral blood mononuclear cells (Supplementary Fig. 2b). For AGER, we noted the presence of two PCR products suggesting an unreported splice variant; we confirmed the presence of the splice variant by sequencing.
