All procedures were done in accordance with the University of Delaware Institutional Animal Care and Use Committee. Long Evans rat litters from timed pregnancies were obtained and litters were culled on postnatal day (PD) 3. Gestational age was used as a reference for the developmental timing of all treatments. Gestational Day (GD) 22 was considered to be the day of birth (PD 0) and thus, PD 4 would be GD 26.
