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Chunk #7 — MATERIALS AND METHODS — Home cage drinking

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Alpha4 subunit-containing GABAA receptors in the accumbens shell contribute to the reinforcing effects of alcohol.
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One week after arrival, rats were habituated to the taste and pharmacological effects of EtOH in their home cages. They received concurrent 24-hour access to both water and EtOH (10% v/v) for 2 weeks, followed by water and EtOH (20% v/v) every other day for 6 weeks. This procedure results in relatively high EtOH intakes (Steensland et al. 2007; Carnicella et al. 2008; Simms et al. 2008).