Here, we observed seven molecularly distinct types of astrocytes with clear regionally specialized distribution. All astrocytes expressed Aqp4, encoding aquaporin 4, the water channel located on astrocyte vascular endfeet. In addition to Bergmann glia of the cerebellum (ACBG), we found olfactory-specific astrocytes (ACOB, unrelated to olfactory ensheathing cells; see below), two subtypes of telencephalon-specific astrocytes (ACTE1 and ACTE2), two subtypes of non-telencephalon astrocytes (ACNT1 and ACNT2), and a Myoc-expressing astrocyte of the dorsal midbrain, ACMB. Müller glia were not observed because we did not sample from the retina.