The SOC procedure was executed in experimental replications composed of either adolescent or adult animals. That is, adults and adolescent rats were run separately, in groups of approximately 8 animals. Adolescent experimental replicas were run sequentially in a 3 month period. A second phase was devoted to the adult experimental replications. A period of approximately 3 months elapsed between these two studies, hence the data for adolescents and adults were analyzed separately. Pharmacokinetic measurements, on the other hand, were conducted simultaneously at both ages, and hence the data were analyzed via between-age analyzes.
