We analyzed whole-exome hybrid capture Illumina sequencing (WES) 36 data from 214 ovarian carcinoma tumor-normal pairs previously analyzed by the TCGA consortium 33. We used the program muTect (K. Cibulskis, et al., in preparation.) We have used a newer version of the program muTect than used in previous analysis of this data 33. The primary improvement in the new version is a reduction in the prior that somatic mutations be at an allelic fraction of 0.5, allowing greater sensitive at low allelic-fraction mutations, such as clonal events in impure samples, or to subclonal mutations. This procedure resulted in 29,268 somatic mutations.
