Neuronal circuits are arranged with high spatial precision and specificity and therefore, spatial information is an important factor in deciphering neuronal activity. Microscopy, fluorescent markers, and transgenic animals have enabled researchers to localize and classify neurons in a high-throughput manner. Together with dynamic multineuron Ca-imaging using spinning-disk confocal microscopy with two-photon excitation, spatial and functional information can be obtained simultaneously. However, the temporal resolution of MEA recordings can capture neuronal responses better than these imaging technologies (Delgado Ruz and Schultz, 2014) and the optical tools described above may not be applicable to all experiments, e.g., due to the unavailability of the transgenic animals, the duration of the experiment, optical access such as in in vivo experiments with freely moving animals, etc. Therefore, localization of neurons in MEA recordings has been of interest for in vivo and acute slice in vitro experiments too.
