Microsatellite genotyping started well before standard genome survey sets of markers were available, and therefore markers were drawn from a variety of sources [2]. Initially data were generated manually using agarose gels; later genotyping switched to automated DNA sequencers (ABI373, ABI377). Allele frequencies were estimated with the USERM13 program [25] and the CRIMAP program [26] were used to estimate marker order and distances. Maps were generated from these data.
