Normalized gene expressions (reads per kilo base per million, RPKM) of 53 tissue types were obtained from GTEx (Supplementary Table 3). A total of 56,320 genes was available in GTEx, which we filtered on an average RPKM per tissue greater than or equal to 1 in at least one tissue type. This resulted in transcripts of 28,520 genes, of which 22,146 were mapped to entrez ID (see “Gene Mapping” section for details). In the GENE2FUNC, the heatmap of prioritized genes displays two expression values; (i) the average log2(RPKM+1) per tissue per gene, in which RPKM is winsorized at 50, allowing comparison of expression level across genes and tissue types and (ii) the average of the normalized expression (zero mean of log2(RPKM+1)) per tissue per gene allowing comparison of expression level across tissue types within a gene.
