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Chunk #139 — STAR★Methods — Quantification and Statistical Analysis — Marker gene set discovery

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Molecular Architecture of the Mouse Nervous System.
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Many, even most, of the cell types described in this paper were not previously associated with known makers. We therefore designed an algorithm to automatically propose marker sets for all clusters. Here, we define a marker gene set as a set of genes that are all expressed in a given cluster, but not all expressed in any other cluster. We used trinarization to judge if a gene is expressed or not in each cluster.