Chunk #42 — EXPERIMENTAL PROCEDURES — RNA isolation, RT-qPCR, and Primers

Source

IRE1α induces thioredoxin-interacting protein to activate the NLRP3 inflammasome and promote programmed cell death under irremediable ER stress.

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RNA was isolated from whole cells using either the Qiagen RNeasy kit (#74104), or the ZR RNA Mini-prep kit (Zymo Research, #R1064). See Supp methods for details on primer sequences and quantitative PCR.