Chunk #25 — RESULTS — Reductions in responding for ethanol after GABAAR α4 subunit knockdown are present when testing occurs at the time of maximal knockdown and require ethanol reinforcement
the NAc shell made significantly fewer active lever responses as compared to control rats [main effect of time (F(5,70) = 34.11, P < 0.001), virus treatment (F(1,70) = 18.11, P < 0.001), treatment by time interaction (F(5,70) = 2.67, P < 0.05)]. Responding by Ad-shα4-treated rats did not differ from Ad-NSS-treated rats during the first 5 minutes, but was significantly decreased 5–10 minutes (P < 0.05) and 10–15 minutes (P < 0.05) after the session began. Similarly, no significant difference was observed in ethanol consumption among the Ad-shα4-treated (0.27 ± 0.036 g/kg) and the Ad-NSS (0.27 ± 0.021 g/kg) rats during the first 5 minutes of the session.
