Details of Flag-ZBTB16, CFP-ZBTB16, His-SUMO1/2/3, HA-UBC9 and SENP1 have been reported previously22,62. Plasmid pLV-mTurquoise2-IL-1β-mNeonGreen was a gift from Dr Hao Wu (Harvard Medical School, Addgene number 166783). HA-ASC and Flag-ASC plasmids were gifted by Dr Eicke Latz (Institute of Innate Immunity, University of Bonn). Mutants of ASC were generated by PCR-mediated, site-directed mutagenesis. PCR primers for gene cloning and site-directed mutagenesis are listed in Supplementary Table S2.
