Chunk #43 — Methods — Measurement of cytokines

Source: Inflammasome activity is controlled by ZBTB16-dependent SUMOylation of ASC.
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Supernatants from cell culture, peritoneal lavage fluid and serum were collected and measured by ELISA as per the manufacturer’s instructions: Mouse IL-1β (eBiosciences); Mouse IL-18 and TNFα and human IL-1β (R & D Systems).