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Chunk #44 — Methods — Lactate dehydrogenase assay

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Inflammasome activity is controlled by ZBTB16-dependent SUMOylation of ASC.
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The release of lactate dehydrogenase (LDH) from cells was measured by LDH Cytotoxicity Assay according to the manufacturer’s instructions (Beyotime Biotechnology). Measurements were performed in duplicate.