Microglia and astrocyte clusters were further divided into four and two subclusters, respectively, by performing another iteration of FindNeighbors and FindClusters on these individual clusters, using cells from the 143 samples. Subcluster-specific genes were determined by using the "roc" test within the FindMarkers function in Seurat (see https://satijalab.org/seurat/reference/findmarkers). The top 50 genes (based on myAUC statistic) were used as input into g:Profiler for each subcluster to determine enriched biological pathways specific to that subcluster.
