Despite the lack of apparent uniformity, a shared finding among the three separate analyses was the differential expression of structural genes, proteins, and phosphorylated proteins and an enrichment in BP related to cellular organization and structure. Many of these genes and proteins are concurrently associated with the synapse CC which was significantly enriched in both the proteomics and phosphoproteomics analyses. The hub proteins analysis of the proteome network discovered vesicle-associated membrane protein-3 and synaptobrevin homolog YKT6 which belong to the SNARE family proteins involved in the synaptic vesicle cycle in the presynaptic terminal (Schwarz 2013). In addition to these two hub proteins, numerous other genes, proteins, and phosphorylated proteins involved in presynaptic vesicle loading, docking, fusion, and neurotransmitter release emerged from our analyses as differentially expressed between lines. These included members of the RIMS family and associated binding proteins (Rims1, Rims2, Rimbp2), synaptotagmins (Syt3 and Syt12), syntaxins and related binding proteins, (Stx1b and Stxbp5l), synaptogyrin-1 (Syngr1), bassoon (Bsn), piccolo (Pcl), and amphiphysin (Amph). Differences in the expression or phosphorylation state of these proteins would be expected to alter presynaptic release
