Chunk #63 — Online Methods — RNA-seq Integration and Visualization

Source

Integrated Single-Cell Multiomic Profiling of Caudate Nucleus Suggests Key Mechanisms in Alcohol Use Disorder.

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To visualize all cells in the same plot, we used functionality in the Seurat v5 and BPCells packages to convert each pool to an on-disk BPCells matrix.96 This allowed us to merge each object in a memory-efficient way. After merging, the function RunUMAP was run on the combined object for 2D visualization.