Two large clusters corresponded to dSPNs (Cluster 10, n=30,835 cells) and iSPNs (Cluster 11, n=25,305)(Striatum dataset, Figure 7A,B) and were distinguished by 68 differentially expressed genes, including known and undescribed pathway markers (Figure 7C). To identify cells from patch and matrix, we inspected transcriptional signals from SPN subclustering (dSPN Cluster 10: n=9 biological ICs; iSPN Cluster 11: n=10, Data S6). Each analysis identified a single candidate “patch IC” whose most strongly contributing genes included known patch markers such as Tac1 and Pdyn. Approximately 10% of iSPNs and dSPNs exhibited this patch signal (Figure S7A,B).
