Plasma was collected from a cohort of fed 14 WT and 28 Tg 15–16 week old male mice. Half of the Tg and all WT mice were sham operated, the remaining Tg groups underwent WT adipose transplantation. Donor WT animals were siblings to recipient Tg males, and sufficient WT epididymal adipose tissue was obtained from a single donor to increase Tg adipose mass by approximately 2 g (maximal amount for successful graft as determined in pilot studies). The adipose tissue was divided into 10 sections of approximately 200 mg each. Two small incisions were made along the ventral midline, and one incision was made on the dorsal side of each recipient. After implant placement, the incisions were closed with surgical glue. Fourteen days post surgery plasma was collected from all animals; the 14 WT mice were randomized into 2 groups such that the average blood glucose and adipose mass levels were similar. This randomization was also done for the Tg sham and Tg transplant groups of mice. Each group was treated with 10 mg/kg body weight/day rmuFGF21 or vehicle BID
