such that the average blood glucose and adipose mass levels were similar. This randomization was also done for the Tg sham and Tg transplant groups of mice. Each group was treated with 10 mg/kg body weight/day rmuFGF21 or vehicle BID for 21 days at which point the mice were necropsied following decapitation. Body composition was determined before, immediately following, and 7, 14, 21 and 28 days after surgery. Fed plasma samples were collected pre-transplant, post-transplant pre-treatment and post-treatment (14th day of treatment), and GTTs were performed on the 18th day of treatment (both 1 hour after dosing). At necropsy, liver, WAT (epididymal, inguinal, and implants) and BAT were excised, weighed and fixed in 10% neutral buffered zinc-formalin, paraffin-embedded, H&E stained and examined by light microscopy.
