Analysis of the expression of pluripotency factors NANOG, SOX2 and OCT4, and cell surface expression of SSEA3, SSEA4, TRA-1–60 and TRA-1–81, and alkaline phosphatase was carried out in immunocytochemistry and polymerase chain reaction (PCR) (Supplementary Figure 1). Controls for pluripotency and differentiation capability include parallel analysis of a well-characterized iPSC line (UM-C2) and H7 or UM4-6 hESC lines.
