Assessment of the tri-lineage differentiation capability of the iPSC lines was done by 45 days of culture in non-adherent dishes in hESC medium lacking FGF2 to form embryoid bodies (EBs). Lineage differentiation was assessed using cell-type restricted antibodies and PCR (Supplementary Figure 1D,Supplementary Tables 1A and B). Ectoderm was identified using nestin or β-III tubulin; mesoderm: Brachyury, smooth muscle actin or decorin; and endoderm: Sox17, alpha-fetoprotein or Foxa2.
