Cerebellar and frontal cortex samples originating from 304 adult individuals of European ancestry, confirmed free of any neuropathological disorder on the basis of histology and examination by a senior neuropathologist, were collected as previously described1,16,20. Total RNA was extracted from sub-dissected samples (100–200 mg) and amplified using the Illumina TotalPrep-96 RNA amplification kit before being directly hybridized onto Human HT12v3 Expression BeadChips (50-mer probes 3′-UTR targeted). The expression data from Illumina Human HT12v3 arrays were analyzed using the Gene Expression Module 3.2.7 in Illumina BeadStudio. Raw intensity values for each probe were transformed using the cubic spline normalization method and then log2 transformed. We remapped the annotation for probes according to ReMOAT60 onto Human Genome Build 19 and restricted the analysis to genes that were reliable, uniquely hybridized, associated with gene descriptions and free of common polymorphisms (MAF >1%). Before use in eQTL analyses, the expression data were corrected for gender, age, post-mortem interval and batch effects (hybridization batch effects and brain bank) as described previously. We successfully mapped ~40% of the Affymetrix transcript IDs to Illumina probe IDs using
