To confirm the function of INCENP in CSCs, we extracted genes that were directly associated with INCENP in the LUAD stem cell gene set. Then we constructed a protein-protein interaction (PPI) interaction network using STRING, and conducted a Biological Process (Gene Oncology, GO) enrichment analysis (Figure 4C). The well-known function of INCENP is associated with centromeres, which was responsible for many kinetochore functions, including microtubule binding, motor activity, and cell cycle signaling (Ainsztein et al., 1998). The enrichment analysis results also indicated that the role of INCENP in CSCs was mainly attributable to the cell cycle.
