Since the proteins of the ZDHHCs family all have the function of catalyzing palmitoylation, and ZDHHC5 is not the only ZDHHC that is highly expressed in LUAD, we asked why only ZDHHC5 was associated with poor prognosis in LUAD. It is widely established that palmitoylated proteins are (almost) always localized at the membrane (except ZDHHC23) and palmitoylation influences protein trafficking between the cytomembrane and the cytosol (or Golgi apparatus). However, over the years, several nuclear proteins have been identified as targets of palmitoylation. Further, INCENP is also a nuclear protein, which suggests a potential role for ZDHHC5 in the control of INCENP in the nucleus. In order to determine the subcellular localization of ZDHHCs, we predicted the ZDHHCs nuclear localization signals (NLSs) specific to the importin αβ pathway using cNLS Mapper (Figure 5A). Briefly, a GUS-GFP reporter protein fused to an NLS with a score of 8, 9, or 10 indicates exclusive localization to the nucleus; scores of 7 or 8 indicate partial localization to the nucleus; a score of 3, 4, or 5 indicates localization to both the nucleus
