an NLS with a score of 8, 9, or 10 indicates exclusive localization to the nucleus; scores of 7 or 8 indicate partial localization to the nucleus; a score of 3, 4, or 5 indicates localization to both the nucleus and the cytoplasm; and scores of 1 or 2 localization to the cytoplasm. We identified 11 ZDHHCs predicted to be localized in the nuclear compartment and the cytoplasm (scores between 3 and 5). To verify these in silico results, we obtained immunofluorescence images of 20 ZDHHC proteins in human cell lines from the HPA database (Supplementary Table 2). We found that ZDHHC5, ZDHHC8, ZDHHC12, ZDHHC14, ZDHHC15, ZDHHC16, and ZDHHC23 localized in the nucleus (Supplementary Figure 3). Figure 5B showed that ZDHHC5 was mainly localized to the plasma membrane and nucleoplasm in A549, in A431 (an epidermoid carcinoma cell line), and in U-2 OS (an osteosarcoma cell line). There were 4 ZDHHCs predicted and verified to be located in nucleus, including ZDHHC5, ZDHHC14, ZDHHC16, and ZDHHC23.
