long constructs include approximately 3000 bp of the putative promoter region of CHRNB3 (common, mixed, and minor), while the shorter pairs cover approximately 1500 bp (short common and short minor). Plasmid preparations from bacterial cultures were done using FastPlasmid Mini-prep kit (Eppendorf, Westbury, NY) for small scale DNA preps or the Qiagen Plasmid Maxi Purification Kit (Qiagen) for large scale preps.
