Prior to injection with saline or ethanol, animals were habituated to the testing apparatus for a period of two hours (exactly as described above for Locomotor activity). Animals were then injected with either saline or ethanol and locomotor activity was recorded for an additional 30 minutes at one minute intervals. One week later, the test was repeated, reversing the saline and ethanol injections. Injection order was counterbalanced by genotype. Separate cohorts of animals were used for each dose of ethanol (1.0, 1.5 and 2.0 g/kg) such that each animal received only a single injection of ethanol and a single injection of saline.
