To investigate the different impacts of intermittent ethanol exposure on the transcriptomic profiles, we compared gene expression profiles with and without ethanol exposure (0 mM versus 75 mM) (table S1). We identified 3169 DEGs associated with intermittent ethanol exposure in high-PRS microglial cells and 2472 DEGs in low-PRS microglial cells (Fig. 4, A and B). Among these DEGs, 1601 were up-regulated in high-PRS microglial cells, while 1308 were up-regulated in low-PRS microglial cells after intermittent ethanol exposure, with 909 genes overlapping between high-PRS and low-PRS microglial cells (fig. S5). GO analysis unveiled significant enrichment of terms from the up-regulated DEGs in high-PRS lines, but not in low-PRS lines, in biological processes related to peptide antigen assembly with the MHCII protein complex and antigen processing and presentation (Fig. 4, C and D). Subsequently, we plotted running scores from gene set enrichment analysis (GSEA) explicitly focusing on the GO biological process terms unique to high-PRS microglial cells. In addition to the terms related to antigen processing and presentation via the MHCII protein complex, this analysis uncovered significant enrichment of terms related to
