Lastly, we used S-MultiXcan v0.7.0 (an extension of S-PrediXcan v0.6.2 [22]) to identify specific eQTL-linked genes associated with POU. This approach uses genetic information to predict transcript abundance in 13 brain tissues, and tests whether the predicted transcripts correlate with POU. S-PrediXcan uses pre-computed tissue weights from the Genotype-Tissue Expression (GTEx) v8 project database (https://www.gtexportal.org/) as the reference transcriptome dataset. For S-PrediXcan and S-MultiXcan analyses, we chose to use sparse (elastic net) prediction models, which are available at http://predictdb.hakyimlab.org/. We applied a conservative Bonferroni correction based on the total number of gene-tissue pairs tested (14,159 gene-tissue pairs tested; p < 3.53E−06).
