In our study, we randomly picked one iPSC line per donor on the basis of recent studies demonstrating that the genetic background of individual donors has greater influence on the transcriptome than line-to-line differences or the reprogramming method used [61]. However, we cannot exclude the significance of line-to-line variability in modeling diseases using iPSCs. Even though the variability between lines might not be as significant as the variability coming from each donor, inclusion of multiple lines might be beneficial to reduce technical errors in future experiments.
