Microglia were missing from our study of isogenic MECP2 wild type and mutant human ES-derived neural cells 26, yet their involvement in Rett syndrome has become in recent year the focus of great interest 27, following controversial findings that transplanted wild-type phagocytes derived from bone marrow could rescue features of Rett syndrome 28. pMGLs have the potential to clarify some of the cell autonomous and non-cell autonomous aspects of Rett microglial biology. We derived pMGLs from isogenic male wild-type and MECP2 mutant cells, and observed that mutant cells were significantly smaller than the wild-type cells (Fig. 3c).
