The average allele ratio from all genomic DNA samples was used as a correction factor for all genomic DNA and cDNA allele ratios, since this could be assumed to reflect a perfect 1:1 ratio of the two alleles and could therefore be used to correct for any inequalities in allelic representation specific to the assay (Bray et al., 2003). T-tests were performed using the average corrected allele ratio for each genomic DNA and cDNA sample. The t-tests were two-tailed with unequal variance, and p values <0.05 were considered to be significant. The real time PCR was performed in triplicate for each cDNA sample and the allelic imbalance ratio was expressed as Aquantity / Tquantity.
