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Chunk #127 — ONLINE METHODS — HBCC replication microarray cohort

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Gene expression elucidates functional impact of polygenic risk for schizophrenia.
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Microarray-based gene expression data were available from the DLPFC samples of the Human Brain Collection Core (HBCC, http://www.nimh.nih.gov/labs-at-nimh/research-areas/research-support-services/hbcc/human-brain-collection-core-hbcc.shtml). These samples were prepared by extraction of RNA using Qiagen RNAeasy kits, generation of biotin-labeled cRNAs using Affymetrix 3’IVT express kits, and hybridization of cRNAs to Illumina HumanHT-12_V4 Beadchips. Expression values were extracted with GenomeStudioV2011.1. After preprocessing and quality control to check for outliers and sex mismatches, there remained 131 SCZ and 176 control samples (as well as 43 BP and 88 MDD samples, where these latter samples were used only for eQTL derivation).