Microarray-based mRNA expression analysis in patient and control-derived neurospheres was conducted to understand the transcriptome profile relevant to schizophrenia with a 22q11.2 deletion. Of genes that are mapped in the deleted region of 22q11.2, expression levels of 22 genes including DGCR8 were significantly decreased in patient-derived neurospheres (Supplementary Table 4). The genome-wide results depicted 263 upregulated and 123 downregulated genes with a 2-fold cutoff value and P<0.05 (Supplementary Figure 4). The differentially expressed genes were mainly enriched for gene ontology terms relevant for cell differentiation, neuronal development and microRNA processing (Supplementary Table 5). Further analysis revealed that upregulated genes were significantly enriched for MAPK (mitogen-activated protein kinase)-mediated nuclear events, neurotransmitter receptor binding, transmission across chemical synapses, nerve growth factor signaling and NMDA receptor activation (Supplementary Table 6). The downregulated genes fell into classes associated mainly with cell cycle-related events (Supplementary Table 6).
