C. elegans homogenates were tested for ethanol concentration according to the manufacturer's directions using an Alcohol Reagent Kit (Pointe Scientific, Canton, MI, USA). 1.5 μL of worm homogenate was added to 300 μL ice-cold alcohol reagent, incubated for five minutes at 30°C, and the reaction was stopped by putting the tube into an ice-cold aluminum block. Alcohol concentration was determined by measuring the absorbance at 340 nm.
