22 h even up to 100 mM ethanol; we chose 75 mM to be within this range and to offer a good possibility of discerning effects. Cells were cultured in the absence or presence of 75 mM ethanol for 24 h, at which time cells were harvested and lysed with buffer RLT, supplied in the Qiagen RNeasy kit, and RNA extractions were conducted per the manufacturer's protocol.
