When comparing the air- and vapor-exposed ethanol consumption (g/kg) for the four post-dependence induction period self-administration sessions that occurred during acute withdrawal (see Figure 2), the two-way ANOVA identified a main effect of Exposure condition (F (1, 8) = 20.445, p≤0.01) and an Exposure × Session interaction (F (3, 24) = 3.813, p≤0.05).Thus, the air- and vapor-exposed groups were different from each other and ethanol vapor-exposure resulted in significantly escalated ethanol self-administration. To provide an example of the rates of responding for the dependent and nondependent animals, the number of lever-presses necessary to achieve the mean levels of ethanol intake (g/kg) during the 4th post-dependence induction acute withdrawal self-administration session (see Figure 2) were 70 (± 3.84) for the vapor-exposed and 26 (± 3.04)for the air-exposed animals.
