The DW and coexpression network data were integrated to focus on those nodes that are hubs in only one of the selected lines and show a significant change in affected edges. Two hundred and fifty-three genes met these criteria (Table S7). Two modules, greenyellow (P < 10−7) and lightcyan (P < 3 × 10−3), were significantly enriched in affected genes; the brown module showed a trend (P > 0.08) toward significant enrichment. Of the affected hub nodes in the greenyellow and lightcyan modules (N = 65), only two genes had a higher intramodular connectivity in the low line (Gm12356 and Dpp6), and both were found in the lightcyan module. The effects of selection specifically on the greenyellow hub node Oprd1 and more generally on intramodular connectivity are illustrated in Fig. 3. Connectivity in the low line is reduced; for Oprd1, the decrease in connectivity was marked (0.88 to 0.46; see Table S7).
