It was of interest to note that among the affected hub nodes in the greenyellow and lightcyan modules, 13 of the nodes were non-coding RNAs, largely antisense to protein-coding genes. One of these non-codes (Gm26672) is antisense to a gene family of the protocadherins described above and includes Pcdhga2, a5, a6, a7b2, b5 and b7. The EnrichR tool (Chen et al. 2013; Kuleshov et al. 2016) was used to determine if the greenyellow and lightcyan hub nodes were significantly enriched in transcription factor (TF) and/or micro-RNA (mi-RNA)-binding sites. No significant enrichment was detected for the greenyellow hubs; however, for the lightcyan module hubs there was a significant enrichment (corrected P < 0.01) in TCFAP2A, ZBT7A and NFKB1 TF-binding sites.
