Differential chromatin accessibility analysis between individuals with and without AUD was performed for each cell type using DESeq2 with the default parameters. Sex, age, and ethnic origin were included as covariates. Genes with FDR < 0.05 (corrected for multiple-hypothesis testing using the BH method) were deemed significant. Regions residing in promoter regions of genes were determined using the R package ChIPSeeker87. The function annotatePeak() was used, with parameters: TxDb = TxDb.Hsapiens.UCSC.hg38.knownGene, annoDb = “org.Hs.eg.db,” and tssRegion = c(−1000, 1000).
