Immunohistochemistry demonstrated an increase in the number of BP neurons expressing the MGE marker NKX2-1, whereas EMX2 was expressed by more control neurons. iPSC remained sensitive to patterning factors as demonstrated by treatment with the Hedgehog pathway activator purmorphamine or to LiCl. Interestingly, preliminary data also demonstrate that this early patterning is maintained with differentiation, with BP neurons expressing higher levels of somatostatin than controls. Considerable research remains to be done to determine the timing and extent to which these alterations are maintained.
