3xTg-AD and WT mice were sacrificed 1month after alcohol or saccharin drinking (as described above) for qualitative assessment of pTau-Ser199/202 (pTau) immunoreactivity (IR). pTau-Ser199/202 IR was not observed in WT mice (Fig. 7A). By contrast, alcohol produced pronounced hyperphosphorylation of Tau-Ser199/202 IR in dorsal hippocampal neuronal cell bodies and projections of 3xTg-AD mice as compared to saccharin controls (Fig. 7A). No difference was observed in BLA of 3xTg-AD mice (Fig. 7B).
