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Chunk #34 — Experimental Procedures — Reagents

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A Nurr1/CoREST pathway in microglia and astrocytes protects dopaminergic neurons from inflammation-induced death.
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All smart-pool siRNAs and GIPZ lentivirus shRNAmir were purchased from Dharmacon and Open Biosystems, respectively. LPS E. coli 0111:B4 used at 0.1 μg/ml final, SB216763 as indicated, and Cycloheximide used at 10 μg/ml final were obtained from Sigma. Human and mouse IL1β used at10 ng/ml final and TNFα used at 50 ng/ml final were from R&D system.