Gene-based tests of associations in MAGMA25 identified 35 genes genome-wide significantly associated with lifetime cannabis use (see Figure 3, Table 2, Supplementary Figure S4, and Supplementary Table S3). These genes were located in 5 regions that were already identified in the SNP-based analysis (including those containing CADM2 and NCAM1) and in 11 different regions (Supplementary Figure S5).