In the analytic sample (n=1,167), we found no evidence that the cannabis PRS was associated with a binary measure of lifetime cannabis use (p = 0.111), nor with frequency of use at baseline (in ever-users, p = 0.390), frequency of use at last assessment (in ever-users, p = 0.513), or maximum frequency of cannabis use (in ever-users, p = 0.090). However, the cannabis use PRS was associated with lifetime history of DSM5 CUD (p = 0.028) but was no longer significant in the subset of ever-users (p = 0.090, Table S4). The pattern of association with cannabis use was similar when individuals with <3 assessments (n=1,840) were studied, although in this larger sample the PRS was associated with maximum frequency of cannabis use (p = 0.013), and with DSM5 cannabis use disorder in both the full sample (p = 0.005) including in ever-users (n = 1,144, p = 0.014).
