To determine if the ethanol sedation assay described here was influenced by the same genetic manipulations that alter ethanol sensitivity in other studies, we analyzed the behavior of several previously described mutants with altered ethanol sensitivity. Ethanol sensitivity in our sedation assay was increased in scb and aru mutants, decreased in Alk and hppy mutants, and decreased in flies with knockdown of NPF (Figure S9), consistent with previous reports (Bhandari et al., 2009; Corl et al., 2009; Eddison et al., 2011; Lasek et al., 2011; Wen et al., 2005). Sensitivity to ethanol sedation in the assay described here seems to be influenced by the same or similar mechanisms as reported for other behavioral paradigms.
