Gene expression profiling was successful for 56 out of 60 samples. RIN scores summarize the distribution of molecular weights and low RIN scores may confound further analyses. All four samples that failed showed a very low RIN score. Due to the use of a different purification method, we had no RIN scores available for the LCLs. Five successfully arrayed samples with high reproducibility between visits showed RIN scores between 1.5 and 6.5 (see Additional file 1). Hierarchical clustering showed however that isolation method was the major response variable and not RIN, yield, individual, chip, detection score or visit.
