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Chunk #16 — Material and Methods — Neuroimaging measures

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Impulsiveness and insula activation during reward anticipation are associated with genetic variants in GABRA2 in a family sample enriched for alcoholism.
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Whole-brain blood oxygen level-dependent (BOLD) functional images were acquired on a 3.0 Tesla GE Signa scanner (Milwaukee, WI) using a T2*-weighted single-shot combined spiral in/out sequence 40 with the following imaging parameters: repetition time (TR) = 2000 ms, echo time (TE) = 30 ms, flip angle (FA) = 90; field-of-view (FOV) = 200 mm; matrix size = 64 × 64; in plane resolution = 3.12 × 3.12 mm; and slice thickness = 4 mm. A high resolution anatomical T1 scan was obtained for spatial normalization (three-dimensional spoiled gradient recalled echo [3-DSPGR], TR=25 msec, min TE, FOV=25 cm, 256×256 matrix, slice thickness=1.4 mm). Participant motion was minimized with the use of foam pads placed around the head along with a forehead strap. In addition, the importance of keeping as still as possible was emphasized during the Informed Consent process and at scanner entry.